Full Length Single Cell RNA Sequencing

Core Technology & Workflow

Using microfluidic digital droplets and Barcoded Beads coupled with random primers, single-cell partition and random capture of the whole transcriptome can be achieved in a step of generating water-in-oil droplets. This convenient process requires only a small amount of sample. The inclusion of an rRNA blocker enables efficient depletion of rRNA, delivering higher proportion of effective data.

1-8 samples can be ﬂexibly run at a time
500-12,000 cells can be captured with a single channel
150,000 emulsion droplets can be generated in 3 minutes
Recovery of ~1000 cells with doublet rates of ~0.3% per 1,000 cells
Even coverage of the full-length of the gene from 5' to 3'end
Flexibility to incorporate a panel for detecting target genes with high

Product Features

Accurate single-cell transcriptome:	Achieve full-length transcriptome detection without being limited by the 3' or 5' end.
Genotype to phenotype:	Simultaneously obtain single-cell mutation and gene expression information, establishing the relationship between genotype and phenotype.
Capturing non-coding RNA:	In addition to mRNA, non-coding RNA without 3' polyA tails can also be captured.
Crossing the species barrier:	Enable the detection of viruses and prokaryotes.
Improving analysis accuracy:	Allow the detection of isoform switching events of mRNA and IncRNA.
Achieving effective rRNA depletion:	The inclusion of an rRNA blocker enables efficient depletion of rRNA

Product Brochure